Xavier Mateos Ferre
Rosa Maria Solé Cartañá
Universitat Rovira i Virgili
Nanoscience, Materials and Chemical Engineering
Detection and quantification of toxins in food by a new opto-microfluidics biosensor. Design and development.
Toxins in food are at the focus of the Health Department in many countries due to the negative effects in humans and animals. The presence and quantification of such toxins in food is monitored by complex, expensive and time-consuming procedures, so that new cost-effective and faster technologies are searched while keeping the detection level and confidence required.
The idea to be developed in the present project is to use a mid-infrared (MIR) laser source that is coupled into a low-loss channel waveguide (enhanced light confinement) whose evanescent field (enhanced sensitivity) interacts selectively by resonant absorption with the specific antibody of the desired toxin flowing in a microfluidic channel with microliter levels of needed samples. The resulting changes in the transmission of the MIR laser source at the output of the channel waveguide are going to be used for sensing.
This novel approach is believed to be a promising tool in toxin screening to guarantee food safety and to minimize potential risks to human and/or animal health.
This work is to be carried out in a multidisciplinary team.
To this aim, the student will fabricate, characterize and functionalize the channel waveguide. The material used will be Silicon-compatible with the aim to be coupled in a Silicon chip for mass production. The micro-structuring of the channel waveguide will be carried out by diamond dicing. In addition, the work will include the assembling of the microfluidic channel on top of the channel waveguide with the required dimensions for the injection and biorecognition of the analyte. Finite Element simulations of the propagating laser mode will be studied to optimize the channel waveguide dimensions, suitable refractive index contrast and evanescent field distribution, as well as the optimization of the microfludic channel.
Finally, the biosensor will be applied to detect and quantify a set of toxins. Two further strategies to enhance the sensitivity of the detection will be applied. Pre-concentration techniques of the analyte as well as electronic amplification of the signal after the propagation along the channel waveguide.
37.5 hours a week
|This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 713679|